Structural characterization and antitumor activity of a polysaccharide from Dendrobium wardianum

Through hot water extraction, protein removal and chromatographic purification, DWPP-Is was found to be the major polysaccharide present in the stem of D. wardianum. The Mn and Mw of DWPP-Is were 29.0 kDa and 98.6 kDa, respectively. Furthermore, mannose and glucose were found to be the most abundant monosaccharides in DWPP-Is. Their backbones consist of (1 → 4)-β-d-Glcp and O-acetylated (1 → 4)-β-d-Manp, which are similar to the structures of other anti-tumour Dendrobium polysaccharides. The inhibition rate of DWPP-Is treatment on SPC-A-1 cells (2 mg/mL, 72 h) reached 56.0%. Intragastric administration of DWPP-Is on A549 tumour-bearing KM mice (10 mg/mL, 0.2 mL) exhibited similar inhibition ratios to that of erlotinib hydrochloride (2 mg/mL). Moreover, the highest inhibition was observed in P-CK treatment combined with DWPP-Is, reaching an inhibition rate of 23.4%. These results suggest that DWPP-Is has the potential to be a functional agent for lung cancer prevention.