细胞生物学
信使核糖核酸
生物
细胞器
生物化学
基因
作者
Weiliang Zhao,Chunxi Zeng,Jiajie Yan,Shi Du,Xucheng Hou,Chengxiang Zhang,Wenqing Li,Binbin Deng,David W. McComb,Yonger Xue,Diana D. Kang,Yizhou Dong
标识
DOI:10.1002/adma.202103131
摘要
Abstract Organelles are specialized compartments, where various proteins reside and play crucial roles to maintain essential cellular structures and functions in mammalian cells. A comprehensive understanding of protein expressions and subsequent localizations at each organelle is of great benefit to the development of organelle‐based therapies. Herein, a set of single or dual organelle labeling messenger RNAs (SOLAR or DOLAR) is designed as novel imaging probes, which encode fluorescent proteins with various organelle localization signals. These mRNA probes enable to visualize the protein localizations at different organelles and investigate their trafficking from ribosomal machinery to specific organelles. According to the in vitro results, SOLAR probes show organelle targeting capabilities consistent with the design. Moreover, DOLAR probes with different linkers display distinct targeting properties depending on different organelle localization signals. Additionally, these mRNA probes also exhibit organelle labeling ability in vivo when delivered by lipid nanoparticles (LNPs). Therefore, these mRNA‐based probes provide a unique tool to study cell organelles and may facilitate the design of organelle‐based therapies.
科研通智能强力驱动
Strongly Powered by AbleSci AI