菁
光漂白
光防护
化学
荧光团
光化学
荧光
猝灭(荧光)
组合化学
生物化学
光合作用
物理
量子力学
作者
Yasser Gidi,Jorge Ramos-Sánchez,Terri C. Lovell,Viktorija Glembockyte,Irwin K. Cheah,Martin J. Schnermann,Barry Halliwell,Cosa Gonzalo
标识
DOI:10.26434/chemrxiv-2023-dxtt7
摘要
Preventing fluorophore photobleaching and unwanted blinking is crucial for singlemolecule fluorescence studies. Reductants achieve photoprotection via quenching excited tripletstates, yet either require counteragents, or for popular alkyl-thiols, are limited to cyanine dye Cy3 protection. Here we provide mechanistic and imaging results showing that the naturally occurring amino acid ergothioneine and its analogue dramatically enhance photostability for Cy3, Cy5 and their conformationally restrained congeners, providing a biocompatible universal solution for demanding fluorescence imaging.
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