The effect of various essential oil and solvent additives on the botanical pesticide of Piper Aduncum essential oil on formulation antifungal activity

This study aims to obtain a formulation of the botanical pesticide P aduncum with additives and the right type of solvent. The research was arranged in a completely randomized design in factorial, the first factor being essential oil additives; citronellagrass oils (Andropogon nardus), lemongrass oils (Cimbopogon flexiosus), Ceylon cinnamon leaf oils (Cinnamomum zeylanicum), Padang cinnamon leaf oils (Cinnamomum burmanii), clove leaf oils (Eugenia aromatica) and wild ginger leaf oils (Elettariopsis slahmong), both types of solvent (ethanol, methanol and turpentine). The experiment was carried out at the Laing Solok IPPTP parasitology laboratory. The results showed that all additive materials had a positive effect in increasing the antifungal activity of the botanical pesticide P aduncum essential oil with different effectiveness against the test fungi. Citronella oil showed the highest antifungal effectiveness compared to other additives, especially against S. rolfsii and Pestalotia sp, with diameter and biomass inhibition of fungal colonies tested S rolfsii 92.15% and 92.70%, Pestalotia sp 84.82% and 86,34% and F. oxysporum 43.87 and 47.17%. All solvents can be used for the formulation of the botanical pesticide P aduncum, ethanol solvent is better than methanol and turpentine with the highest inhibition capacity of the diameter and biomass of the fungus colonies tested S rolfsii 91.28% and 92.05%, Pestalotia sp 83.56% and 86.02% and for F. oxysporum 40.31 and 48.36%, respectively.