聚腺苷酸
夜蛾
生物
夜蛾科
裂解和多聚腺苷酸化特异性因子
转录组
遗传学
抄写(语言学)
核糖核酸
生殖器鳞翅目
植物
基因
基因表达
重组DNA
语言学
哲学
作者
Liying Fang,Lei Guo,Min Zhang,Xianchun Li,Zhongyuan Deng
出处
期刊:Insects
[MDPI AG]
日期:2022-09-02
卷期号:13 (9): 803-803
标识
DOI:10.3390/insects13090803
摘要
During the messenger RNA (mRNA) maturation process, RNA polyadenylation is a key step, and is coupled to the termination of transcription. Various cis-acting elements near the cleavage site and their binding factors would affect the process of polyadenylation, and AAUAAA, a highly conserved hexamer, was the most important polyadenylation signal (PAS). PAS usage is one of the critical modification determinants targeted at mRNA post-transcription. The full-length transcriptome has recently generated a massive amount of sequencing data, revealing poly(A) variation and alternative polyadenylation (APA) in Spodoptera frugiperda. We identified 50,616 polyadenylation signals in Spodoptera frugiperda via analysis of full-length transcriptome combined with expression Sequence Tags Technology (EST). The polyadenylation signal usage in Spodoptera frugiperda is conserved, and it is similar to that of flies and other animals. AAUAAA and AUUAAA are the most highly conserved polyadenylation signals of all polyadenylation signals we identified. Additionally, we found the U/GU-rich downstream sequence element (DSE) in the cleavage site. These results demonstrate that APA in Spodoptera frugiperda plays a significant role in root growth and development. This is the first polyadenylation signal usage analysis in agricultural pests, which can deepen our understanding of Spodoptera frugiperda and provide a theoretical basis for pest control.
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