A Critical Review of Conventional and Modern Approaches to Develop Herbicide‐Resistance in Rice

Abstract Together with rice, weeds strive for nutrients and space in farmland, resulting in reduced rice yield and quality. Planting herbicide‐resistant rice varieties is one of the effective ways to control weeds. In recent years, a series of breakthroughs have been made to generate herbicide‐resistant germplasm, especially the emergence of biotechnological tools such as gene editing, which provides an inherent advantage for the knock‐out or knock‐in of the desired genes. In order to develop herbicide‐resistant rice germplasm resources, gene manipulation has been conducted to enhance the herbicide tolerance of rice varieties through the utilization of techniques such as physical and chemical mutagenesis, as well as genome editing. Based on the current research and persisting problems in rice paddy fields, research on the generation of herbicide‐resistant rice still needs to explore genetic mechanisms, stacking multiple resistant genes in a single genotype, and transgene‐free genome editing using the CRISPR system. Current rapidly developing gene editing technologies can be used to mutate herbicide target genes, enabling targeted genes to maintain their biological functions, and reducing the binding ability of target gene encoded proteins to corresponding herbicides, ultimately resulting in herbicide‐resistant crops. In this review article, we have summarized the utilization of conventional and modern approaches to develop herbicide‐resistant cultivars in rice as an effective strategy for weed control in paddy fields, and discussed the technology and research directions for creating herbicide‐resistant rice in the future.