血小板
Percoll公司
凝集素
欧洲乌列克斯
化学
分子生物学
细胞生物学
生物
生物化学
免疫学
离心
凝集素
作者
DaMatta Ra,Lauro Manhães,Seabra Sh,de Souza W
出处
期刊:PubMed
日期:1998-04-01
卷期号:22 (1): 45-52
被引量:9
摘要
Chicken leukocytes were separated from blood on a Percoll cushion following adherence on coverslips, resulting in a coculture of thrombocytes and monocytes. This system was characterized by light microscopy, scanning and transmission electron microscopy, by lectin binding and actin localization in thrombocytes. During the first 24 hours nuclear condensation, cytoplasmic shrinkage, detachment and death of thrombocytes were observed. In contrast, monocytes gradually increased their spreading capacity, specially after thrombocyte detachment from the coverslips. During culture, a large number of fucose and beta-galactose residues were expressed on the surface of thrombocytes, revealed by the lectins Ulex europaeus I and Arachis hypogaea, respectively. Labeling of the monocyte surface with several lectins also increased with the cultivation time. Thrombocytes showed the formation of a net with actin involvement.
科研通智能强力驱动
Strongly Powered by AbleSci AI