安培法
氨基酸
生物传感器
苯丙氨酸
过氧化氢
化学
甘氨酸
色谱法
D
亮氨酸
氧化酶试验
电解质
电极
生物化学
酶
电化学
物理化学
作者
Priyabrata Sarkar,Ibtisam E. Tothill,Steven Setford,Anthony P. F. Turner
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:1999-01-01
卷期号:124 (6): 865-870
被引量:111
摘要
Screen-printed three-electrode amperometric sensors incorporating L- and/or D-amino acid oxidase for the general purpose measurement of L- or D-amino acids is described. The working electrode incorporates rhodinized carbon, to facilitate hydrogen peroxide oxidation at a decreased operating potential, and immobilized enzyme. The devices responded to all 20 common L-amino acids and all of the D-amino acids examined, the exceptions being L- and D-proline. Linear response profiles were observed for L-leucine, L-glycine and L-phenylalanine with limits of detection of 0.47, 0.15 and 0.20 mM respectively. The devices were reproducible and exhibited stability over a 56 d test period. The biosensor compares favourably with a standard photometric amino acid test and was used to monitor milk ageing effects. The assay is cheap, simple to perform and rapid, requiring only buffer-electrolyte and a small sample volume.
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