Chemical constituents of Physalis alkekengivar.franchetii(II)

Objective To investigate the chemical constituents in persistent calyx of Physalis alkekengi var.franchetii.Methods Various colume chromatographies over silica gel,Sephadex LH-20,and HPLC were used to separate the chemical constituents and their structures were elucidated by physicochemical property,1H-NMR,13C-NMR,and MS.Results Four chemical constituents were obtained and identified as caffeic acid ethyl ester(1),25,27-dehydro-physalin L(2),physalin D(3),and cuneataside E(4).Conclusion Compound 2 is a new natural product,and compound 4 is isolated from the plant for the first time.