The β-FeOOH crystallite were prepared with chitosan using urea neutralization boiling forcing-hydrolysis method.The influence of chitosan on β-FeOOH crystallite were studied.It is found that β-FeOOH long axis diameter increases with increasing amount of chitosan and decreases with the rising pH.Chitosan is being think as β-FeOOH crystallite growing promoters when pH4.2 and as inhibitors when pH4.2. β-FeOOH is also characterized by TEM,XRD and IR in this paper.It is found that the external diameter of subcrystals of β-FeOOH is in 1~4nm range.The formation process of β-FeOOH with chitosan is being preliminary deduced.