Simultaneous determination of Itaconic Acid and Saccharides in Itaconic Acid Fermentation Broth of Aspergillus terreus by High Performance Liquid Chromatography

A high performance liquid chromatographic method for rapid determination of itaconic acid and saccharides in itaconic acid fermentation broth of Aspergillus terreus was developed. A TOSOH Tskgel o Apak-A column was used at 45 ℃ and the mobile phase was 0. 75 mmol / L H2SO4 at a flow rate of 1. 0 m L / min. Under the optimized conditions. The itaconic acid and glucose in samples were determined and completely separated within 20 min by a refractive index detector( RID). The Linear correlation coefficient for itaconic acid was 0. 999 9 in the range of 0 ~ 10. 056 g / L. The Linear correlation coefficient for glucose was 0. 999 9 in the range of 0 ~ 6. 016 g / L. The relative standard deviation( RSD,n = 6) of itaconic acid and glucose was 0. 33% and 0. 15% respectively. The recovery rate was 100. 88% ~ 102. 74% and of 101. 26% ~ 103. 12% separately. This method established is proved to be fast and accurate for the quantitative analysis of the itaconic acid and glucose in fermentation broth of Aspergillus terreus.