Constructing a NIR fluorescent probe for ratiometric imaging viscosity in mice and detecting blood viscosity in folliculitis mice and peritonitis mice

For a variety of viscosity-related diseases, studying blood viscosity may be an ever so direct, simple, non-invasive as well as effective pre-diagnostic method. At present, the main cause for the insufficiently thorough studies on blood viscosity is the lack of tools that can not only self-calibrate but also shield the interference of bio-auto fluorescence from blood. Herein, we have judiciously designed and synthesized a new fluorescent probe Nile-TPE to tackle the above problem. This TBET structure based fluorescent probe possess numerous merits including near-infrared (NIR) emission, ratiometric response, lipid droplets positioning, wide emission peak spacing, etc. Besides the viscosity ratiometric imaging in vitro, probe Nile-TPE also realizes the ratiometric imaging viscosity in mice and detecting blood viscosity in peritonitis mice and folliculitis mice for the first time. And, importantly, this effective method requires only 10 μL of blood. Therefore, probe Nile-TPE furnishes a more accurate tool for non-invasive diagnosis of viscosity-related diseases.