A Rapid Method for the Purification of Immunoglobulin M (IgM) from the Sera of Certain Mammalian Species

Abstract The purification of normal IgM is typically accomplished using a combination of euglobulin precipitation, gel filtration and electrophoresis or ion-exchange chromatography. The euglobulin fraction of serum contains many macroglobulins, including IgM, and is readily precipitated from serum by dialysis against distilled water1. Mandena et al.2 and Martin3 have published data that relate the solubility of IgM to ionic strength. Solubility is also affected by pH and temperature4. Separation of IgM from most other euglobulins may be accomplished by gel filtration4, chromatography on DEAE cellulose5, and zonal electrophoresis on starch, Pevikon6 or agar7.