Nucleotide Sequence of the "Denaturable" Leucine Transfer RNA from Yeast

The nucleotide sequence of "denaturable" leucine acceptor transfer RNA (tRNA Leu 3 ) from baker's yeast was determined on 32 P-labeled material. The molecule is 85 nucleotides long and can be folded into the "cloverleaf" model for secondary structure. The basis on which the sequence was deduced from the products of complete enzymatic digestion, prior to its unambiguous determination, is presented.