气凝胶
纳米纤维
聚己内酯
明胶
静电纺丝
脚手架
生物相容性
材料科学
组织工程
化学
纳米技术
生物医学工程
复合材料
聚合物
医学
生物化学
冶金
作者
Yongyao Tan,Dan Chen,Yun-Ming Wang,Wei Wang,Lingjuan Xu,Rong Liu,Chunxiu You,Guigang Li,Huamin Zhou,Dequn Li
标识
DOI:10.1002/mabi.202100441
摘要
Constrained by the existing scaffold inability to mimic limbal niche, limbal bio-engineered tissue constructed in vitro is challenging to be widely used in clinical practice. Here, a 3D nanofiber-aerogel scaffold is fabricated by employing thermal cross-linking electrospinned film polycaprolactone (PCL) and gelatin (GEL) as the precursor. Benefiting from the cross-linked (160 °C, vacuum) structure, the homogenized and lyophilized 3D nanofiber-aerogel scaffold with preferable mechanical strength is capable of refraining the volume collapse in humid vitro. Intriguingly, compared with traditional electrospinning scaffolds, the authors' 3D nanofiber-aerogel scaffolds possess enhanced water absorption (1100-1300%), controllable aperture (50-100 µm), and excellent biocompatibility (optical density value, 0.953 ± 0.021). The well-matched aperture and nanostructure of the scaffolds with cells enable the construction of limbal bio-engineered tissue. It is foreseen that the proposed general method can be extended to various aerogels, providing new opportunities for the development of novel limbal bio-engineered tissue.
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