胰岛素受体底物
胰岛素受体
IRS2
污渍
胰岛素
胰岛素样生长因子1受体
IRS1
受体
GRB10型
化学
生物
细胞生物学
胰岛素抵抗
生物化学
内分泌学
生长因子
基因
作者
Jinghua Peng,Balamurugan Ramatchandirin,Alexia Pearah,Liang He
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2023-01-01
卷期号:13 (4)
标识
DOI:10.21769/bioprotoc.4619
摘要
Far-western blotting, derived from the western blot, has been used to detect interactions between proteins in vitro, such as receptor-ligand interactions. The insulin signaling pathway plays a critical role in the regulation of both metabolism and cell growth. The binding of the insulin receptor substrate (IRS) to the insulin receptor is essential for the propagation of downstream signaling after the activation of the insulin receptor by insulin. Here, we describe a step-by-step far-western blotting protocol for determining the binding of IRS to the insulin receptor.
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