Effect of Tryptanthrin Compound on Lipopolysaccharide-Induced Inflammation Model in L929 Cell Line

Objective: Our aim is to evaluate the effects of the tryptanthrin compound, with antimicrobialand anti-inflammatory effects, on the lipopolysaccharide inflammation model created in theL929 cell line.Methods: L929 cell lines were placed in a 5% CO2 incubator at 37°C, using standard cell cultureprocedures, and lipopolysaccharide was applied with Prn pure substance with a final concentrationof 2 μL and 1 μL after 1 hour. Following the lipopolysaccharide application, the (3- [4,5- dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium bromide protocol, a colorimetric method, was applied todetermine cell viability at the end of the required 24th, 48th, and 72nd hours incubation times.Results: IC50 values were calculated as 0.3 μg/mL for tryptanthrin and 1 μg/mL for lipopolysaccharide. Application of lipopolysaccharide to L929 cell lines caused a significant decrease in cellularindex depending on time. Also, in the tryptanthrin + lipopolysaccharide groups, it was foundthat the decreased cell index significantly increased even closer to the control compared to thelipopolysaccharide applied group. It was found that the inflammation and cell damage caused bylipopolysaccharide applied to the L929 cell line improved after tryptanthrin application.Conclusion: When we look at the results of our study as a whole, it was determined that theviability rates of the cells were above 85% as a result of the tryptanthrin application and it was significantlyabove the 70% viable cell ratio recommended by ISO 10993-5: 2009 Dın en ISO (2009)for cytotoxicity.