Laser – fluorescence based evaluation of remineralization in artificial enamel lesions using three commercially available re-mineralizing agents – An invitro study

The objective of this in-vitro study is evaluation of remineralization potential of three remineralization agents- Tricalcium phosphate flouride(TCP-F), Calcium sucrose phosphate(CaSP), Casein phosphopeptide- amorphous calcium phosphate(CPP-ACP) in artificial enamel lesion using laser fluorescence based DIAGNOdent(KaVo). 30 single rooted premolars were selected. Samples were randomly divided into 3 groups with 10 samples each. Baseline values were recorded before any intervention. A 4mmx4mm window was prepared on the buccal surface which was then subjected to demineralization for 96 hr at 37°C. DIAGNOdent values were recorded after demineralization. Remineralization was done using Group I TCP-F, Group II CaSP, Group III CPP-ACP remineralizing agents. The samples were subjected to DIAGNOdent analysis after 72hrs, 7days, 14 days. The data was analyzed with analysis of variance (ANOVA) and Post Hoc test. All three groups showed significant difference in DIAGNOdent values at baseline, post demineralization and post remineralization. TCP-F group showed maximum remineralization followed by CaSP and CPP-ACP groups. DIAGNOdent is a reliable non-invasive caries detecting device to measure early demineralization. TCP-F, CaSP, CPP-ACP are proven to possess remineralization properties.