Dual dye-labeled aptamers for detection of dichlorvos using ratiometric fluorescence resonance energy transfer

Dichlorvos (DDVP) is an efficient and highly toxic organophosphorus pesticide. Considering its effects on human health and ecosystems, pesticide residue and pollution monitoring is of great significance. Traditional methods like chromatography with mass spectrometry are not portable or rapid because they use large instruments and complex pre-processing methods. Compared with other optional on-site detection technologies, like enzymatic and antibody methods, aptamers are advantageous because they are stable, readily modified, and inexpensive. Therefore, screening and developing a specific adapter for DDVP detection is necessary and will be of practical value.