化学
生物合成
环肽
立体化学
组合化学
生物化学
肽
酶
作者
Maxine Treisman,Laura Coe,Yongwei Zhao,Vishnu Mini Sasi,Jemma Gullick,Mathias Henning Hansen,A. M. Ly,Victor Leichthammer,Caroline Heß,Daniel L. Machell,Ralf B. Schittenhelm,Joel F. Hooper,Colin J. Jackson,Julien Tailhades,James J. De Voss,Max J. Cryle
标识
DOI:10.1021/acs.orglett.3c04366
摘要
Cytochrome-P450-mediated cross-linking of ribosomally encoded peptides (RiPPs) is rapidly expanding and displays great potential for biocatalysis. Here, we demonstrate that active site engineering of the biarylitide cross-linking enzyme P450Blt enables the formation of His-X-Tyr and Tyr-X-Tyr cross-linked peptides, thus showing how such P450s can be further exploited to produce alternate cyclic tripeptides with controlled cross-linking states.
科研通智能强力驱动
Strongly Powered by AbleSci AI