Photostable pH-Sensitive Near-Infrared Aggregation-Induced Emission Luminogen for Long-Term Mitochondrial Tracking

Mitochondria are crucial in the process of oxidative metabolism and apoptosis. Their morphology is greatly associated with the development of certain diseases. For specific and long-term imaging of mitochondrial morphology, we synthesized a new mitochondria-targeted near-infrared (NIR) fluorescent probe (TPE–Xan–In) by incorporating TPE with a NIR merocyanine skeleton (Xan–In). TPE–Xan–In displayed both absorption (660 nm) and emission peaks (743 nm) in the NIR region. Moreover, it showed aggregation-induced emission properties at neutral pH and specifically illuminated mitochondria with good biocompatibility, superior photostability, and high tolerance to mitochondrial membrane potential changes. With a pH-responsive unit, hydroxyl xanthene (Xan), the probe exhibited a pH-sensitive fluorescence emission in the range of pH 4.0–7.0, which indicated its potential in long-term tracking of pH and morphology changes of mitochondria in the biomedical research studies.