Research on a highly sensitive aptamer sensor for Vibrio alginolyticus based on CRISPR-Cas13a and T7 transcription cascade amplification

). When applied to simulated seawater and seafood samples, the recovery rates ranged from 94.61% to 106.56%, indicating robust anti-interference capacity and reproducibility. This work establishes a highly sensitive and specific biosensing technology for the on-site rapid detection of aquatic pathogens, offering promising applications in environmental monitoring and food safety.