生物
蛋白质组
表型
转录组
细胞生物学
蛋白质组学
计算生物学
膜蛋白
亚细胞定位
心肌细胞
人类蛋白质组计划
生物信息学
病理
基因表达
基因
医学
遗传学
膜
细胞质
作者
Shin‐Haw Lee,Sina Hadipour-Lakmehsari,Da Hye Kim,Michelle Di Paola,Uroš Kuzmanov,Saumya Shah,Joseph Jong-Hwan Lee,Thomas Kislinger,Parveen Sharma,Gavin Y. Oudit,Anthony O. Gramolini
标识
DOI:10.1038/s41597-020-00762-1
摘要
Abstract In the current study we examined several proteomic- and RNA-Seq-based datasets of cardiac-enriched, cell-surface and membrane-associated proteins in human fetal and mouse neonatal ventricular cardiomyocytes. By integrating available microarray and tissue expression profiles with MGI phenotypic analysis, we identified 173 membrane-associated proteins that are cardiac-enriched, conserved amongst eukaryotic species, and have not yet been linked to a ‘cardiac’ Phenotype-Ontology. To highlight the utility of this dataset, we selected several proteins to investigate more carefully, including FAM162A, MCT1, and COX20, to show cardiac enrichment, subcellular distribution and expression patterns in disease. We performed three-dimensional confocal imaging analysis to validate subcellular localization and expression in adult mouse ventricular cardiomyocytes. FAM162A, MCT1, and COX20 were expressed differentially at the transcriptomic and proteomic levels in multiple models of mouse and human heart diseases and may represent potential diagnostic and therapeutic targets for human dilated and ischemic cardiomyopathies. Altogether, we believe this comprehensive cardiomyocyte membrane proteome dataset will prove instrumental to future investigations aimed at characterizing heart disease markers and/or therapeutic targets for heart failure.
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