乌司他丁
医学
KEAP1型
肾
免疫印迹
炎症
枸杞
脂多糖
NF-κB
败血症
药理学
内科学
病理
化学
生物化学
转录因子
基因
替代医学
作者
Qi Wu,Lantao Liu,Xueyong Wang,Zhifang Lang,Xiangjun Meng,Shu Guo,Bin Yan,Tao Zhan,Hua Zheng,Hongwei Wang
出处
期刊:Life Sciences
[Elsevier]
日期:2020-02-01
卷期号:242: 117240-117240
被引量:32
标识
DOI:10.1016/j.lfs.2019.117240
摘要
Lycium barbarum polysaccharides (LBP) are derived from Wolfberry and have antioxidant activities. This study aimed to evaluate the efficacy of LBP for kidney injury in a rat model of sepsis. Male rats were divided randomly to control group (Con), LPS group (LPS), ulinastatin group (ULI), low dose LBP group (LBP-1), middle dose LBP group (LBP-2) and high dose LBP group (LBP-3). After intraperitoneal injection of LPS (5 mg/kg) to make sepsis model (LPS group), 10,000 U/kg ulinastatin were given in ULI group, and 200, 400 and 800 mg/kg LBP was given in LBP-1, -2, -3 group, respectively. Serum IL-1β, IL-6, IL-8, TNF-α and NF-κB levels were measured by ELISA. Nrf2, Keap1, NF-κB, HO-1 and NQO1 expression levels were detected by PCR and Western blot analysis. We found that LBP decreased the levels of NF-κB and pro-inflammatory cytokines while attenuated kidney injury. In addition, LBP regulated Keap1-Nrf2/ARE signaling pathway in the kidney. In conclusion, LBP attenuates inflammation injury in the kidney via possible regulation of Keap1-Nrf2/ARE signaling.
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