Engineering of E. coli inherent fatty acid biosynthesis capacity to increase octanoic acid production

BACKGROUND: existing metabolic network, with the objective of improving bio-octanoic acid production. RESULTS: expression, medium pH, and C:N ratio resulted in the identified strain producing 500 mg/L of C8 and 805 mg/L of total FAs, an 82 and 155% increase relative to wild-type MG1655 (TE10) in shake flasks. The best engineered strain produced with high selectivity (> 70%) and extracellularly (> 90%) up to 1 g/L free octanoic acid in minimal medium fed-batch culture. CONCLUSIONS: for other customized bioproducts.