钙
神经油
钙显像
光度测定(光学)
神经科学
生物物理学
生物
医学
物理
内科学
中枢神经系统
星星
天文
作者
Alex A. Legaria,Bridget A. Matikainen‐Ankney,Ben Yang,Biafra Ahanonu,Julia A. Licholai,Jones Griffith Parker,Alexxai V. Kravitz
标识
DOI:10.1038/s41593-022-01152-z
摘要
Fiber photometry enables recording of population neuronal calcium dynamics in awake mice. While the popularity of fiber photometry has grown in recent years, it remains unclear whether photometry reflects changes in action potential firing (that is, 'spiking') or other changes in neuronal calcium. In microscope-based calcium imaging, optical and analytical approaches can help differentiate somatic from neuropil calcium. However, these approaches cannot be readily applied to fiber photometry. As such, it remains unclear whether the fiber photometry signal reflects changes in somatic calcium, changes in nonsomatic calcium or a combination of the two. Here, using simultaneous in vivo extracellular electrophysiology and fiber photometry, along with in vivo endoscopic one-photon and two-photon calcium imaging, we determined that the striatal fiber photometry does not reflect spiking-related changes in calcium and instead primarily reflects nonsomatic changes in calcium.
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