Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic

A novel peroxidase-mimicking DNAzyme-based colorimetric homogenous bioassay is developed for kanamycin detection. Aptamer biorecognition triggers DNAzyme liberation from DNA duplexes for "off-on" signal transduction. The special hairpin structure of the aptamer-kanamycin biocomposite enables Exo III-assisted target recycling for signal amplification.