化学
适体
核酸
检出限
生物分子
安培法
组合化学
DNA
生物传感器
核酸检测
杂交探针
分析物
纳米颗粒
核酸热力学
寡核苷酸
电化学
电极
纳米技术
生物化学
色谱法
分子生物学
基序列
物理化学
生物
材料科学
作者
Ronen Polsky,Ron Gill,Lubov Kaganovsky,Itamar Willner
摘要
Nucleic acid-functionalized Pt nanoparticles (Pt-NPs) act as catalytic labels for the amplified electrochemical detection of DNA hybridization and aptamer/protein recognition. Hybridization of the nucleic acid-modified Pt-NPs with a sensing nucleic acid/analyte DNA complex associated with an electrode enables the amperometric, amplified, detection of the DNA by the Pt NP electrocatalyzed reduction of H2O2 (sensitivity limit, 1 × 10-11 M). Similarly, the association of aptamer-functionalized Pt- NPs to a thrombin aptamer/thrombin complex associated with an electrode allowed the amplified, electrocatalytic detection of thrombin with a sensitivity limit corresponding to 1 × 10-9 M.
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