生物
GenBank公司
多重聚合酶链反应
聚合酶链反应
多路复用
16S核糖体RNA
欧洲联盟
遗传学
基因
业务
经济政策
作者
Alessandra Dalmasso,Enrico Fontanella,Piergiovanni Piatti,Tiziana Civera,Sergio Rosati,Maria Teresa Bottero
标识
DOI:10.1016/j.mcp.2003.09.006
摘要
A multiplex Polymerase Chain Reaction (PCR) assay was applied to feedstuff analysis for the identification of the most used species in rendering plants (ruminant, poultry, fish and pork materials). Primers were designed in different regions of mitochondrial DNA (12S rRNA, tRNA Val and 16S rRNA) after alignment of the available sequences in the GenBank database. The primers generated specific fragments of 104–106, 183, 220–230 and 290 bp length for ruminants, poultry, fish and pork, respectively. The detection limit was 0.004% for fish primers and 0.002% for ruminants, poultry and pork primers. The multiplex PCR proposed in this study can be considered a valid alternative to the microscopic method for the detection of animal derived materials banned by a European Union Regulation as a preventive measure against the spread of Bovine Spongiform Encephalopathy.
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