A Bioluminogenic Substrate for In Vivo Imaging of β‐Lactamase Activity

Graphical Abstract Reporting live: A caged β-lactam–D-luciferin (Bluco) conjugate can image β-lactamase activity in vivo through a two-step enzymatic process. This novel bioluminogenic probe should facilitate the detection of β-lactamase and widen its applications as a reporter.