电致变色
细菌
半胱氨酸
谷胱甘肽
抗生素
抗菌剂
聚合物
金黄色葡萄球菌
材料科学
组合化学
革兰氏阴性菌
微生物学
化学
生物化学
生物
大肠杆菌
电极
酶
复合材料
物理化学
基因
遗传学
作者
Jiayingzi Wu,Yifan Zhu,Lijun You,Pu‐Ting Dong,Jianguo Mei,Ji‐Xin Cheng
标识
DOI:10.1002/adfm.202005192
摘要
Abstract The electrochromism of a water‐soluble naturally oxidized electrochromic polymer, ox‐PPE, is harnessed for rapid and facile bacterial detection, discrimination, and susceptibility testing. The ox‐PPE solution shows distinct colorimetric and spectroscopic changes within 30 min when mixed with live bacteria. For the underlying mechanism, it is found that ox‐PPE responds to the reducing species (e.g., cysteine and glutathione) released by metabolically active bacteria. This reduction reaction is ubiquitous among various bacterial strains, with a noticeable difference that enables discrimination of Gram‐negative and Gram‐positive bacterial strains. Combining ox‐PPE with antibiotics, methicillin‐susceptible and methicillin‐resistant Staphylococcus aureus can be differentiated within 2.5 h. Proof‐of‐concept demonstration of ox‐PPE for antimicrobial susceptibility testing is carried out by incubating Escherichia coli with various antibiotics. The obtained minimum inhibition concentrations are consistent with the conventional culture‐based methods, but with the procedure time significantly shortened to 3 h.
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