Extraction of Jatropha curcas seed protein

Alkaline extraction and acid precipitation were employed to produce protein from Jatropha curcas seed meal.The optimized conditions were obtained by orthogonal test as follows:alkaline extraction solid-liquid ratio 1∶11,pH 8.5,temperature 55℃,time 100 min;acid precipitation pH 4.4,temperature 55℃,time 40 min.Under the conditions,the extraction rate of Jatropha curcas seed protein was 76.71%,and the purity of protein reached 81.18%.