Establishment of mice acute liver injury model induced by alcohol and its application

Objective To establish the acute alcoholic liver injury model and apply in evaluation for health care foods. Methods Mice were randomized divided into five groups (4 dosage groups and one control group) and given 50%v alcohol. The four dosage groups were executed after fasting 6,8,10,12 hours respectively. Liver samples were observed pathologically with frost slice techniques and Sudan Ⅲ staining. The evaluation of liver function protection effects followed the national standard (2003). Results The pathological change of the test groups was fatty degeneration, fasting 12 hours group were remarkably different from the control group (P0.01). The fatty degeneration grades and the mean ranks of these two groups were 22 and 12, and 27.5 and 9.79 respectively. The samples tested had certain liver protection function using this model. The fatty degeneration grade in every group was less than the positive control (P0.05). MDA contents decreased significantly in low and high dosage groups (P0.05). GSH-PX contents in all test groups were increased significantly (P0.05 or P0.01).Conclusion Mice acute liver injury model can be developed by given one dose of 50%v alcohol and fasted for 12 hours and can be applied in evaluating the liver protection of health care foods.