Development of TiO2/Bi2O3/PANI as a novel glucose biosensor and antimicrobial agent

TiO2/Bi2O3/PANI nano-composite materials were synthesized, characterized and examined towards biological applications. Different assays were conducted to determine the effect of the synthesized materials on the bacteria (gram-positive and gram-negative bacteria). The results show that reactive oxygen species induced by the nanomaterials disrupt the microbial cellular structure and destroy the cell membrane. Quorum sensing inhibition assay or anti-biofilm assay shows the potential activity of nanoparticles as quorum sensing inhibitors and potential anti-biofilm agents. The synthesized materials were also employed as a biosensor for the detection of glucose. These nanoparticles not only provide effective support for the immobilization of the glucose oxidase (GOD) enzymes in redox reactions but also facilitate the electron communication between the electrode surface and hosted protein. TiO2/Bi2O3/PANI composite showed a good response signal in the glucose solution. The linear behavior (with correlation coefficient around 1) of anodic and cathodic peak current with the square root of scan rate was confirmed the quasi-reversible surface confined process. The obtained results reveal that the synthesized ternary nanocomposites possess good antimicrobial activity and biosensing properties and have promising applications in medical and clinical diagnostics analysis.