毛细管电泳
残余物
DNA
凝胶电泳
色谱法
荧光
分子生物学
化学
生物
生物化学
计算机科学
光学
算法
物理
作者
Xuan Shen,Xiaomu Chen,David E. Tabor,Lunzhao Yi,Methal Albarghouthi,Yifan Zhang,Mark S. Galinski
出处
期刊:Biologicals
[Elsevier]
日期:2013-05-01
卷期号:41 (3): 201-208
被引量:10
标识
DOI:10.1016/j.biologicals.2012.12.001
摘要
Residual host cell DNA poses potential safety concerns for cell culture-derived vaccines or other biological products. In addition to the quantity of residual DNA, the size distribution is an important measure for determination of its associated risk factor. We have developed a new method for residual DNA size analysis, based on capillary gel electrophoresis (CGE) technology with sensitive laser induced fluorescence detection (LIF). The performance of this method was optimized through empirical selection of appropriate testing conditions and optimized conditions are presented. Examples are given to demonstrate the successful employment of this method for residual DNA size analysis of cell culture-produced vaccine samples.
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