Enantiomeric separation ofd-/l-norepinephrine and -epinephrine by high-performance liquid chromatography with a ?-cyclodextrin type chiral stationary phase

Enantiomeric separation of d-/l-norepinephrine (NE) and d-/l-epinephrine (E) was investigated with various mobile phases by high-performance liquid chromatography using a beta-cyclodextrin type chiral stationary phase as a chiral column. The mobile phase of 2.5 M phosphate buffer (pH 3.0) gave separation factors (alpha s) of 1.04 and 1.06 for NE and E, respectively.