Effect of YKL-40 Transfection on Proliferation and Invasion Activities of Prostate Cancer LNcap Cells

Objective To investigate exogenous YKL-40 gene transfection on the proliferation,invasion,migration and adhesion activities of prostate cancer LNcap cells.Methods The expressions of endogenous YKL-40 gene in prostate cancer DU-145,PC-3 and LNcap cells were determined by RT-PCR.LNcap cells were transfected with plasmid pcDNA3.1-YKL-40 and determined for proliferation,invasion,migration and adhesion activities before and after transfection by MTT method,Boyden chamber method and adhesion test,based on which in vitro drug-sensitivity test was performed.Results Endogenous YKL-40 gene was only expressed in DU-145 cells.On days 2 ~ 5 after transfection with plasmid pcDNA3.1-YKL-40,the A490 values of LNcap cells were significantly higher than those of control(P 0.05).The numbers of cells with invasion and transmembrane migration were(75.11 ± 4.40) and(133.00 ± 5.07) respectively,and the adhesion rate was 107.57%,which were significantly higher than those of control(P 0.05).The IC50 of transfected LNcap cells to 5-FU,cisplatin and etoposide were(31.15 ± 0.43),(4.15 ± 0.13) and(55.22 ± 0.57) μmol / L respectively,which were significantly higher than those of control(P 0.05).Conclusion YKL-40 gene promoted the proliferation and increased the invasion,migration and adhesion activities as well as the resistance to 5-FU,cisplatin and etoposide of LNcap cells.