Dual-target electrochemical aptasensor based on co-reduced molybdenum disulfide and Au NPs (rMoS2-Au) for multiplex detection of mycotoxins

Multiple mycotoxin contamination has posed health risks in the area of food safety. In this study, co-reduced molybdenum disulfide and gold nanoparticles (rMoS2-Au) were designed and used for the first time as an efficient platform endowing electrochemical electrodes with superior electron transfer rates, large surface areas and strong abilities to firmly couple with large amounts of different aptamers. After further modification with thionine (Thi) and 6-(Ferrocenyl) hexanethiol (FC6S), a platform enabling sensitive, selective and simultaneous determination of two important mycotoxins, zearalenone (ZEN) and fumonisin B1 (FB1), was achieved. The established aptasensor showed excellent linear relationships (R2 > 0.99) when ZEN and FB1 concentrations were in the range of 1 × 10−3–10 ng mL−1 and 1 × 10−3–1 × 102 ng mL−1, respectively. High sensitivity of ZEN and FB1 with a limit of detection as low as 5 × 10−4 ng mL−1 was obtained with excellent selectivity and stability. The effectiveness of the aptasensor was verified in real maize samples, and satisfactory recoveries were attained. The established platform could be easily expanded to other aptamer-based multiplex screening protocols in biochemical research and clinical diagnosis.