神经酰胺
二酰甘油激酶
生物化学
磷酸化
磷脂酰甘油
磷脂酸盐
胶束
脂质信号
化学
激酶
脂肪酸
色谱法
磷脂
膜
生物
酶
蛋白激酶C
磷脂酰胆碱
有机化学
水溶液
细胞凋亡
作者
Paul P. Van Veldhoven,W. Robert Bishop,D A Yurivich,R M Bell
出处
期刊:PubMed
日期:1995-05-01
卷期号:36 (1): 21-30
被引量:11
摘要
The phosphorylation of ceramide solubilized in octylglucoside/phosphatidylglycerol mixed micelles by E. coli diacylglycerol kinase was evaluated. Ceramide containing non-hydroxy fatty acids appeared to be phosphorylated quantitatively over a broad range from 25 to 2000 pmoles. If a 2-hydroxy fatty acid was present in the ceramide molecule, phosphorylation was not quantitative. When applied to cellular lipid extracts, TLC of the phosphorylated products is needed to separate ceramide-phosphates from the other labelled compounds (i.e. phosphatidate and lysophosphatidate) and revealed the presence of ceramides containing long and very long chain fatty acids. The mass levels of these ceramides in different cultured cells varied between 0.2 to 0.6 mol% (normalized to phospholipids). Changes in these levels were observed under different stress conditions such as heat treatment or addition of DMSO or detergents to the cell cultures.
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