清脆的
基因组编辑
计算生物学
基因
生物
引导RNA
诱导多能干细胞
遗传学
胚胎干细胞
出处
期刊:Methods in molecular biology
日期:2019-01-01
被引量:7
标识
DOI:10.1007/978-1-4939-9170-9
摘要
This book includes guidelines to select the best CRISPR-Cas system and target sites, covering CRISPR gRNA design, CRISPR delivery and activity quantification, and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening.
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