丙酮
化学
萃取(化学)
盐析
蒸馏
色谱法
盐(化学)
水溶液
下游加工
环境友好型
生物转化
液-液萃取
蒸发
双水相体系
有机化学
生态学
物理
生物
热力学
酶
作者
Xueqi Shi,Muxin Yang,Aqiang Chu,Fengyi Zhang,Jing Fang,Ning Xu,Yanjun Jiang,Hao Li
摘要
Abstract Alpha‐ketoglutaric acid (α‐KG), as an essential intermediate in biosynthesis and drug synthesis, has a broad application prospect. However, the lower product concentration and impurities in the α‐KG production make the downstream separation more complex and costly. In this study, α‐KG was separated from biotransformation broth by salting‐out extraction (SOE) combined with solar‐driven distillation. First, the aqueous two‐phase system (ATPS) consisting of acetone/(NH 4 ) 2 SO 4 was selected by comparison. The effects of acetone/(NH 4 ) 2 SO 4 concentration, temperature, α‐KG concentration, and pH on the distribution behavior of α‐KG in ATPS were investigated. Under the optimized conditions, higher extraction efficiency and purity of α‐KG were obtained in the actual biotransformation broth. In addition, solar evaporation was used to achieve preconcentration of the organic phase and salt recovery, significantly reducing energy consumption. The method is environmentally friendly and easy to operate, providing a new idea for separating low concentration products in biosynthesis.
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