核酸
化学
检出限
DNA
人口
核酸定量
核酸扩增试验
聚合酶链反应
分子生物学
病毒学
计算生物学
生物
生物化学
基因
色谱法
医学
环境卫生
沙眼衣原体
作者
Dewen Kong,Xuejun Wang,Cheng Gu,Mingquan Guo,Yao Wang,Zhaolin Ai,Shen Zhang,Yiheng Chen,Wentao Li,Yungen Wu,Changhao Dai,Qianying Guo,Di Qu,Zhaoqin Zhu,Youhua Xie,Yunqi Liu,Dacheng Wei
摘要
Rapid screening of infected individuals from a large population is an effective means in epidemiology, especially to contain outbreaks such as COVID-19. The gold standard assays for COVID-19 diagnostics are mainly based on the reverse transcription polymerase chain reaction, which mismatches the requirements for wide-population screening due to time-consuming nucleic acid extraction and amplification procedures. Here, we report a direct nucleic acid assay by using a graphene field-effect transistor (g-FET) with Y-shaped DNA dual probes (Y-dual probes). The assay relies on Y-dual probes modified on g-FET simultaneously targeting ORF1ab and N genes of SARS-CoV-2 nucleic acid, enabling high a recognition ratio and a limit of detection (0.03 copy μL–1) 1–2 orders of magnitude lower than existing nucleic acid assays. The assay realizes the fastest nucleic acid testing (∼1 min) and achieves direct 5-in-1 pooled testing for the first time. Owing to its rapid, ultrasensitive, easily operated features as well as capability in pooled testing, it holds great promise as a comprehensive tool for population-wide screening of COVID-19 and other epidemics.
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