外消旋混合物
对映体
化学
玻璃碳
检出限
不对称碳
碳纳米纤维
环糊精
铜
电化学
核化学
循环伏安法
立体化学
电极
有机化学
色谱法
催化作用
物理化学
光学活性
作者
Sharad S. Upadhyay,Nayan S. Gadhari,Ashwini K. Srivastava
标识
DOI:10.1016/j.bios.2020.112397
摘要
Stereochemical configuration of the drug is responsible for racemic switch with enantiomers in presence of chiral environment for human beings. Therefore, its determination in racemic and pharmaceutical samples becomes a challenge. Addressing this issue, an enantioselective electrochemical biomimetic sensor for discrimination of isomers of ethambutol (ETB) employing square wave voltammetry (SWV) is reported for the first time. For this purpose, a chiral host, β-Cyclodextrin based copper metal organic framework (CD-CuMOF) was synthesized and used for chelate complexation of ETB isomers (SS-ETB/RR-ETB). A glassy carbon electrode (GCE) is chemically modified using CD-CuMOF and carbon nanofibers (CNF) composite material to construct a sensor in the form of (CD-CuMOF-CNF-GCE). The behaviour of CD-CuMOF for ETB isomers on GCE is postulated to be an artificial enzyme model (AEM) as it mimics the catalytic activity similar to enzyme alcohol dehydrogenase for ETB. The biosensor exhibits excellent peak potential difference (ΔEp (SS-RR) = 108 mV) between ETB isomers using SWV showing a clear distinction in the racemic mixture. It showed a linear response in the range of 1.0 x 10-7 to 1 x 10-4 M and 5.0 x 10-7 to 2.5 x 10-4 M with low detection limit of 3.10 x 10-8 M and 8.52 x 10-8 M for RR-ETB and SS-ETB isomers respectively. The sensor was applied for the estimation of ETB isomers in racemic mixture and real samples viz., blood, urine and pharmaceutical. The CD-CuMOF is a low-cost material with higher stability than enzyme and offers an advantage for sensing and catalysis in future.
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