Simultaneous Determination of Chlorogenic Acid and Rutin Contents in Corn Silk by RP-HPLC

The content of chlorogenic acid and rutin in corn silk was simultaneously determined by RP-HPLC to explore its measuring method.The results showed that the linear range was 0.144~ 0.864 μg(r = 0.999 4) for chlorogenic acid and 0.281~1.686 μg(r = 0.999 2)for rutin,the average recoveries(n = 5) of chlorogenic acid and rutin were 100.8% and 98.6 %,respectively.The method is rapid,accurate with good reproducibility,which can be applied in simultaneous determination of chlorogenic acid and rutin in corn silk.