Research on establishing the detection method of Mycobacterium tuberculosis by inserting sequence IS6110

Objective To establish a sensitive,specific method for detection of Mycobacterium tuberculosis H37 Rv by useing PCR,and discuss the application value in the early detection of Mycobacterium tuberculosis. Methods Design a pair of specific primers base on the inserted sequence IS6110 with high specificity of Mycobacterium tuberculosis. PCR amplification assays were performed for Mycobacterium tuberculosis H37 Rv,E. coli,Salmonella enteritidis,Shigella,S. aureus and Listeria monocytogenes.Results 247 bp fragments were amplified only from Mycobacterium tuberculosis H37 Rv and was not found in all nonmycobateria. Sensitivity test can detect 10 fg mycobacterium tuberculosis DNA. Conclusion Inserting sequence IS6110 PCR application is a sensitive,specific method,which provides a new reference for Mycobacterium tuberculosis detection in early laboratory tests.