Cytokines Production of Murine Spleen Cells Induced by Gene Transfer of 4-1BBL into Hepatocellular Carcinoma Hepa1-6 in Vitro

Objective To study the cytokines production of spleen cells induced in vitro by murine 4-1BBL gene transfected Hepa1-6.Methods Murine hepatocellular carcinoma cell line Hepa1-6-m4-1BBL which could highly express murine 4-1BBL was cultivated previously. The tumor cell vaccine (TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC). Cocultivation TCV with syngeneic murine spleen cells and the supernatants were harvested for detecting the cytokines (IL-2,TNF-α and GM-CSF).Results Comparing with TCV-Hepa1-6, high levels of IL-2,TNF-α and GM-CSF were released by the spleen cells after stimulated by TCV-m4-1BBL.Conclusion Stimulation signals delivered by 4-BBL remarkably increased the production of IL-2,TNF-α and GM-CSF.