Sixty-eight peach cultivars were measured for their anthocyanin content by Vis-UV Spectrophomery and High performance liquid chomatography for comparing the consistency of the two methods and analysis for cultivar genetic diversity,and then suggested a criterion for red-flesh peach classification.The results showed that anthocyanin content in white-flesh peach was very similar by the two methods and we could choose one of them,while only HPLC was suitable for yellow-flesh peach.The major anthocyanin in peach fruit was cyanidin 3-glucoside that was existed in 48 cultivars,while cyanidin 3-rutinoside was identified in some cultivars,such as Wuheijiroutao and Daguoheitao.Grade index and reference cultivars were given by statistical anthocyanin data for red peach description.These grade indexes were recorded on 1-5 scale which classified 20 mg/100 g as critical point.From this study,we indentified five true red-flesh peaches,other cultivars with visual red-flesh and sweet flavor did not conform to the criterion,we would do further study to solve this problem.