Optimization of Shake Flask Fermentation of Astaxanthin-high-producing Strains of Yeasts

In this study,astaxanthin-high-producing strains of yeasts of shake flask culture conditions were optimized using single factor and orthogonal.The results showed that the appropriate carbon and nitrogen source were glucose and ammonium sulfate,the optimal dosage of glucose,ammonium sulfate,dipotassium hydrogen phosphate,yeast extract and tomato juice were 4%、0.4%、0.4%、0.6% and 0.6%,the optimal medium initial pH was 5,the optimal fermentation temperature was 22 ℃,and the optimal liquid volume was 50 mL/500 mL(medium volume/flaskvolume).