Purification and Characterization of Recombinant SARS-CoV Nucleocapsid Protein

Objective To purify and characterize the recom binant SARS-CoV nucleocapsid prote in expressed in E.coli BL21by automated protein chromatog raphy system.Methods Recombinant SARS-CoV nucleocapsid protein expressed in E.coli BL21was analyzed by SDS-PAGE.The expressed product was purified with Hi s·Bind TM affinity chromatography on automated protein chromatography system.Results The expressed recombinant nucleoca psid protein was in supernatant of the lysates with a molecular weight about 49000u.The recombinant protein was purified w ith His·Bind TM affinity chromatography on automated protein chromatography system.T he recombinant SARS-CoV nucleocapsid protein could be detected by SARS patient sera and rabbit sera immunized w ith GST-N in Western blot analysis.Conclusion The recombinant SARS-CoV nucleocap sid protein was successfully expressed and purified,which can be used for diagnosis and vaccination.