蛋白质组学
生物
细胞分化
基因表达谱
计算生物学
T辅助细胞
基因
免疫系统
基因表达
遗传学
T细胞
作者
Henk-Jan van den Ham,Nadine A. Binai,Fatiha Zaaraoui-Boutahar,Albert J. R. Heck,Arno C. Andeweg
出处
期刊:Proteomics
[Wiley]
日期:2019-03-18
卷期号:19 (7)
被引量:1
标识
DOI:10.1002/pmic.201800045
摘要
Helper T cell differentiation is a key process in the regulation of adaptive immune responses. Here, mouse Th1 and Th2 cells are profiled using high-throughput proteomics to increase the understanding of the molecular biology of Th differentiation to support the design of prophylactic and therapeutic intervention strategies for (infectious) diseases. Protein profiling of Th1/Th2 differentiated cells results in the quantification of almost 6000 proteins of which 41 are differentially expressed at FDR < 0.1, and 19 at the FDR < 0.05 level, respectively. Differential protein expression analysis identifies a number of the expected canonical Th differentiation markers, and gene set analysis using the REACTOME database and a hypergeometric test (FDR < 0.05) confirms that helper T cell pathways are the top sets that are differentially expressed. Additionally, by network analysis, many differentially expressed proteins are associated with the Th1 and Th2 pathways. Data are available via PRIDE database with identifier PXD004532.
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